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Document Type |
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Article In Journal |
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Document Title |
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Differential Expression of VEGF and PAI-1 in Oral Squamous CellCarcinoma Differential Expression of VEGF and PAI-1 in Oral Squamous CellCarcinoma |
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Document Language |
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English |
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Abstract |
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Vascular Endothelial Factor [VEGF] is an endothelial cell-specific mitogen that regulates most
steps of the angiogenic process, including endothelial cell degradation of extracellular matrix
(ECM), migration, proliferation, and tube formation. In keeping with its ability to induce ECM
degradation, VEGF increases the expression and activity of plasminogen activators, urokinase
type plasminogen activator (uPA) and tissue type plasminogen activator (tPA). Both proteases
are specifically inhibited by plasminogen activator inhibitor type 1 (PAI-1), the expression of
which is also up regulated by VEGF. Specific Aims: To examine the differences between VEGF
and PAI-1 expression in oral squamous cell carcinoma [SCCs] and normal human oral
keratinocytes [NHOK]. Experimental Design: 1- Cell lines and culture conditions: a- A total of
three human oral cancer cell lines [Cal33, SCC-4 & SCC-9] and NHOK isolated from different
sites of oral mucosa [Tongue, Gingiva, & Palate]. b- Cells were grown to 80% confluence, and
subjected to different conditions, Normoxia [20% O2] and Hypoxia [2% O2]. 2- VEGF & PAI-1
were examined on both translation and transcription levels using the following: a- Northern Blot
Analysis, b- Enzyme-Linked immunoAbsorbant assay [ELIZA], c- Zymography of PAI-1
(Reverse fibrin overlay), d- Western Blot Analysis and e- Immunocytochemistry. Results:
Hypoxia upregulated VEGF and PAI-1 expression on both normal and oral cancer cell lines,
with a statistically significant difference between normal and oral cancer cell lines. Pattern of
hypoxia induced VEGF mRNA level tightly followed the PAI-1 mRNA expression in the cell
lines tested. From the above results, we conclude that oral Squamous cell carcinoma express
VEGF and PAI-1 more than NHOK that are upregulated by hypoxia. VEGF and PAI-1can play a
role in oral cancer angiogenesis, progression and invasion.
The abstract of this paper has been published at Journal of Dental Research and Oral
presentations at International Association of Dental Research [IADR] / March 2002 in San Diego
– CA - USA. |
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ISSN |
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0 |
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Journal Name |
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JDR |
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Volume |
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81 |
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Issue Number |
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1 |
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Publishing Year |
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2002 AH
2002 AD |
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Article Type |
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Article |
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Added Date |
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Monday, February 22, 2010 |
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Researchers
| خالد محمد | Mohamed, khalid | Researcher | Doctorate | |
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